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目的建立一种快速、简便测定保健食品中硫酸软骨素的液相色谱方法,并进行方法学研究。方法样品经过前处理,以乙腈和0.005 mol/L戊烷磺酸钠溶液为流动相,等度洗脱,经Diamonsil C18柱分离,于192 nm波长检测。结果用本方法进行检测,能很好地分离硫酸软骨素,在浓度0.008 mg/m L~0.04 mg/m L之间呈现良好的线性关系,方法的相关系数可达1.000,平均回收率在98.5%~100.0%之间,相对标准偏差为0.6%。结论采用该方法能测定保健食品中硫酸软骨素,耗时短、操作简便、准确、重现性好,分离效果明显,可在实验室推广。
OBJECTIVE To establish a rapid and simple method for the determination of chondroitin sulfate in health food by liquid chromatography and to conduct a methodological study. Methods The sample was pretreated with acetonitrile and 0.005 mol / L sodium pentane sulfonate solution as the mobile phase and eluted with isocratic elution. The sample was separated on a Diamonsil C18 column and detected at 192 nm. The results of this method for the detection of good separation of chondroitin sulfate in the concentration of 0.008 mg / m L ~ 0.04 mg / m L showed a good linear relationship between the correlation coefficient of up to 1.000, the average recovery was 98.5 % ~ 100.0%, the relative standard deviation of 0.6%. Conclusion The method can be used to determine chondroitin sulfate in health food. The method has the advantages of short time-consuming, simple and convenient operation, accurate and good reproducibility, and obvious separation effect, which can be popularized in the laboratory.