为了进一步从基因水平探讨紫外线的损伤机制，我们采用ＲＴＰＣＲ方法，用紫外线照射大鼠角膜，检测大鼠角膜内三种抗氧化酶：谷胱甘肽过氧化物酶（ＧＳＨＰｘ），铜锌超氧化物歧化酶（Ｃｕ、ＺｎＳＯＤ），过氧化氢酶（ＣＡＴ）ｍＲＮＡ的表达。结果显示，照射早期，三种抗氧化酶ｍＲＮＡ表达均升高，ＧＳＨＰｘ和ＳＯＤ的表达高点为照射５ｍｉｎ，ＣＡＴ的表达高点为照射２ｍｉｎ；照射晚期，照射１５ｍｉｎ，三种酶的ｍＲＮＡ表达明显下降（Ｐ＜００５），照射后４８ｈ，三种抗氧化酶的表达基本恢复正常。由此可见，紫外线可影响大鼠角膜内抗氧化酶ｍＲＮＡ的表达，而且角膜有能力修复这种损伤。作为对照的６磷酸葡萄糖脱氢酶Ｇ６ＰＤ在照射前后没有明显的变化 In order to further explore the mechanism of UV damage from the gene level, we use RT  PCR method to irradiate the cornea with ultraviolet light to detect three antioxidant enzymes in the cornea of ​​rat: glutathione peroxidase (GSH  Px) Cu, ZnSOD and CAT mRNA expression were detected. The results showed that in the early irradiation, the expression of three kinds of antioxidant enzymes mRNA were elevated, GSH  Px and SOD expression was 5min irradiation, CAT expression was irradiated for 2min; late irradiation, irradiation 15min, three kinds of enzyme mRNA (P <005). After 48h irradiation, the expression of three antioxidant enzymes returned to normal. Thus, ultraviolet light can affect the rat corneal antioxidant enzyme mRNA expression, and the cornea has the ability to repair this damage. As a control, 6-phosphoglucose dehydrogenase G6PD showed no significant change before and after irradiation