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OBJECTIVE:To examine changes in body weight and the lung inflammation factors interleukin-1β(IL-1β),interleukin-8(IL-8),IL-10 and tumor necrosis factor-α(TNF-α) in a rat model of cold-dryness syndrome in Northwest(Xinjiang) China to provide a reference for treating chronic obstructive pulmonary disease(COPD) with local peculiarities.METHODS:The rat COPD model was established by intratracheal instillation of porcine pancreatic elastase(PPE) in combination with cigarette smoking(CS).The rat model of cold-dryness syndrome of COPD in the northwest of China was set up by intratracheal instillation of PPE in combination with CS and environmental cold-dryness stress.The level of IL-1β,IL-8,IL-10 and TNF-α in Bronchoalveolar lavage fluid(BALF) were measured by enzyme-linked immunosorbent assay(ELISA).The data were analyzed using the software SPSS 11.5.RESULTS:1) Body weight was less in the two model groups than that of control group(P<0.01),PPE plus CS cold-dryness group was less than that of PPE plus CS group(P<0.01).2) IL-1β in BALF significantly increased in PPE plus CS and cold-dryness group than that of control group(P<0.01).3) IL-8 and TNF-α in BALF significantly increased in PPE plus CS and cold-dryness group and PPE plus CS group than that of control group(P<0.01).CONCLUSION:Body weight in COPD model rats was reduced compared with controls.Cold-dryness may aggravate such a condition Lung inflammation in the model was mainly manifested by an increase in IL-1β,IL-8 and TNF-α levels,with no change in IL-10 levels.Cold-dryness may aggravate lung inflammation of COPD.
OBJECTIVE: To examine changes in body weight and the lung inflammation factors interleukin-1β, interleukin-8 (IL-8), IL-10 and tumor necrosis factor-α cold-dryness syndrome in Northwest (Xinjiang) China to provide a reference for treating chronic obstructive pulmonary disease (COPD) with local peculiarities. METHODS: The rat COPD model was established by intratracheal instillation of porcine pancreatic elastase (PPE) in combination with cigarette smoking (CS). The rat model of cold-dryness syndrome of COPD in the northwest of China was set up by intratracheal instillation of PPE in combination with CS and environmental cold-dryness stress.The level of IL-1β, IL-8, IL -10 and TNF-α in Bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). The data were analyzed using the software SPSS 11.5.RESULTS: 1) Body weight was less in the two model groups than that that of control group (P <0.01), PPE plus CS cold-dryness group was less than th (P <0.01) .2) IL-1β in BALF significantly increased in PPE plus CS and cold-dryness group than that of control group in BALF significantly increased in PPE plus CS and cold-dryness group and PPE plus CS group than that of control group (P <0.01) .CONCLUSION: Body weight in COPD model rats was reduced compared with controls. Cold-dryness may aggravate such a condition Lung inflammation in the model was mainly manifested by an increase in IL-1β, IL-8 and TNF-α levels, with no change in IL-10 levels. Cold-dryness may aggravate lung inflammation of COPD.