An experimental study on the testing of process control for a real-time control system is presented. Several indexes, such as the capability of system approximation output, the ramp rates, the smoothness and stability of output, and control of temperature overshoot, are selected as performance parameters. With these indexes, the thermal performance of thermal cyclers is validated, analyzed, and monitored. A testing prototype is designed and fabricated as a supplementary instrument for the experimental study. A tracking temperature algorithm with feedforward and feedback controls are also introduced to improve the efficiency of system performance testing.