论文部分内容阅读
按照实验设计,用食蟹猴疟原虫子孢子感染恒河猴,待原虫血症出现,然后用咯萘啶 6mg/kg、蒿甲醚 10 mg/kg 和氯喹 20 mg/kg 联合治疗 3 d,清除红内期原虫,观察 100 d内的近期复发情况。如复发则再给予联合治疗。接种子孢子50个的猴M192和55×10~2个的猴 M193无近期复发,而接种子孢子 11×10~3 个的猴 M194 于治疗后 47 d复发 1次,接种子孢子 55×10~4 个的猴M 195分别于治疗后 30 d、37 d和 51 d 出现复发。结果显示,接种子孢子数量宜在 11×10~3 个以上。本研究实际上已对前实验研究进行了总的验证,并且实现了总体设计的预定目的,用引自越南的食蟹猴疟原虫建立了近期复发动物模型。
According to the experimental design, rhesus macaques were infected with Plasmodium cynomolgi malariae to be treated with parasitemia and then treated with pyronaridine 6 mg / kg, artemether 10 mg / kg and chloroquine 20 mg / kg for 3 days, Red-stage protozoa were removed and the recent recurrence within 100 days was observed. If the recurrence is given again combined treatment. M192 inoculated with 50 spores and 55 × 10 ~ 2 in M193 showed no recent relapse, and 11 × 10 ~ 3 monkeys M194 inoculated with sporozoites were recurred 47 days after treatment and inoculated with spores of 55 × 10 ~ 4 monkeys M 195 relapse occurred 30 days, 37 days and 51 days after treatment, respectively. The results showed that the number of inoculated sporozoites should be 11 × 10 ~ 3 or more. The present study has in fact carried out a general validation of the pre-experimental studies and has achieved the intended purpose of the overall design with the establishment of a recent recurrent animal model using Plasmodium cynomolgus from Vietnam.