脆弱类杆菌(Bacteroides fragilis)多糖体英膜作为一种毒力决定因素已得到了相当重视,并证实多糖体荚膜能促进腹腔内脓肿的形成。本文报告了一种区分有荚膜与无荚膜脆弱类杆菌的间断性percoll密度梯度法。脆弱类杆菌NCTC9343株、ATCC23745株和NCTC10584株供作试验用。培养方法:将细菌生长在vail Tassell等所规定的肉汤,Deacon等的基础培基或溶化的人血琼脂平板(phtes of lysed human-blood agar, The membrane of Bacteroides fragilis polysaccharides has received considerable attention as a virulence determinant and has demonstrated that polysaccharide capsular membranes promote the formation of intraperitoneal abscesses. This article reports an intermittent percoll density gradient method that distinguishes between capsular and non-capsular Bacteroides fragilis. Bacteroides fragilis NCTC9343 strain, ATCC 23745 strain and NCTC10584 strain for testing purposes. Culture Methods: Bacteria were grown on broth as defined by Vail Tassell et al., Basal cultures of Deacon et al., Or phtes of lysed human-blood agar