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目的：为了使已经石蜡包埋的肾穿组织脱蜡后进行电镜观察，关键是选择适当的固定剂。方法：在石蜡包埋前，肾穿组织分别用甲醛、戊二醛及戊二醛、锇酸双固定。根据光镜和电镜观察结果，选择能兼顾光镜和电镜的固定剂。结果：甲醛固定不能有效保存肾穿组织的超微结构，锇酸的固定能有效地保存超微结构，但严重影响了光镜切片的染色；而戊二醛与甲醛相比，较好地保存了肾穿组织的超微结构，同时又不影响光镜切片的染色。结论：在石蜡包埋前，用戊二醛对肾穿组织进行固定处理，使得利用蜡块组织进行电镜诊断成为可能。另外，二甲苯对蜡块组织的脱蜡完全与否，也是此项实验中影响环氧树脂包埋的一个重要因素。
Objective: In order to deparaffinize paraffin-embedded kidney-worn tissues, the key is to select the appropriate fixative. Methods: Before paraffin embedding, the renal peritoneal tissues were fixed with formaldehyde, glutaraldehyde, glutaraldehyde and osmium tetroxide respectively. According to light and electron microscopy observations, choose to take both light and electron microscopy fixative. Results: Formaldehyde fixation could not effectively preserve the ultrastructure of kidney-penetrating tissue. Osmium tetroxide fixation could effectively preserve the ultrastructure, but severely affected the staining of light microscopy sections. However, glutaraldehyde was better preserved than formaldehyde The ultrastructure of kidney-weaved tissue did not affect the staining of light microscopy sections. CONCLUSION: Before paraffin embedding, fixation of renal penetrating tissue with glutaraldehyde makes it possible to diagnose using electron microscopy using paraffin blocks. In addition, xylene paraffin wax tissue completely or not dewaxing, but also in this experiment affect the embedding of epoxy resin is an important factor.