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本研究采用琼脂平板扩散法从土壤中分离筛选得到8株对番茄灰霉病菌(Botrytis cinerea)具有显著抑菌活性的菌株。离体叶片颉颃实验表明,菌株12-34可显著抑制番茄灰霉病病原菌的生长。通过菌体和菌落形态的观察及生理生化性状分析,初步判断8株颉颃菌为芽孢杆菌属(Bacillus)。对菌株12-34的16S rDNA序列分析表明,该菌株与Bacillus velezensis的16S rDNA序列同源性达99.78%;结合菌株12-34的生理生化特征,将其鉴定为Bacillus velezensis。采用生防相关功能基因srfAA、srfAB、bmyB、fenD、bioA和ituC的特异引物,首次从12-34菌株中扩增到相关的基因片段,表明该菌株具有一定的生防应用潜力。
In this study, eight strains isolated from soil were obtained by agar plate diffusion method. Eight strains with significant antibacterial activities against Botrytis cinerea were obtained. In vitro leaves antagonistic experiments showed that strain 12-34 can significantly inhibit the growth of tomato gray mold pathogen. Through the observation of cell morphology and colony morphology and the analysis of physiological and biochemical characteristics, eight strains of Fungicides were identified as Bacillus. The 16S rDNA sequence analysis of strain 12-34 showed that the 16S rDNA sequence of this strain shared 99.78% identity with that of Bacillus velezensis. The strain 12-34 was identified as Bacillus velezensis according to its physiological and biochemical characteristics. The specific genes of SRF-related genes srfAA, srfAB, bmyB, fenD, bioA and ituC were amplified for the first time from 12-34 strains, indicating that the strain has certain biocontrol potential.