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温敏雄性不育系具有育种程序简单、组配自由、育种周期短等优点,已成为杂种优势利用的重要材料。本研究以芥菜型(Brassica juncea L.)油菜温敏雄性不育系K121S与核不育系1161A获得的BCF1为作图群体,利用混合分组分析法(bulked-segregant analysis BSA)对温敏雄性不育系K121S的育性转换基因(Fc)进行定位。结果表明获得2个基于RAPD的SCAR标记与育性转换基因(Fc)连锁,即是SS503-250和SBA485-482,与育性转换基因的遗传距离分别为3.94 c M和7.09 c M。本研究结果为今后育性转换基因的克隆、测序及生物信息学研究并解析K121S育性转换的分子机制机制提供了条件。
Thermo-sensitive male sterile lines have the advantages of simple breeding program, free distribution, short breeding cycle, etc., and have become important materials for the utilization of heterosis. In this study, BCF1 obtained from Brassica juncea L. thermo-sensitive male sterile line K121S and the male sterile line 1161A was used as a mapping population, and analyzed by bulked-segregant analysis (BSA) Fertility Transform Gene (Fc) of Fertility Line K121S was Localized. The results showed that two RAPD-based SCAR markers were linked to the fertility-transforming gene (Fc), namely SS503-250 and SBA485-482, with genetic distances of 3.94 cM and 7.09 cM, respectively. The results of this study provided conditions for the future cloning, sequencing and bioinformatics studies of fertility transgene genes and the molecular mechanisms of K121S fertility transversion.