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以9个代表性石榴品种为试材,分别采用氧化还原滴定法、称重法及游标卡尺测量法测定了石榴种皮纤维素、木质素含量和种皮厚度,使用硬度计测定籽粒的硬度,并分析了3种指标与石榴籽硬度之间的皮尔森相关系数,以探讨石榴籽硬度的影响因素,为石榴资源开发利用及石榴品种选育提供参考。结果表明:不同石榴品种之间籽粒硬度、种皮纤维素、木质素含量及种皮厚度存在着一定差异;籽粒硬度与种皮纤维素、木质素含量及种皮厚度均呈正相关,相关系数分别为0.936、0.965和0.994,其中种皮厚度与硬度的相关性最显著,其次是木质素含量和纤维素含量;表明石榴籽粒硬度受种皮纤维素、木质素含量以及厚度等多种因素影响,其中种皮厚度是最重要的影响因素。
Nine representative pomegranate cultivars were used as test materials, the content of cellulose and lignin in the seed coat of pomegranate was determined by redox titration, weighed and vernier calipers respectively. The hardness of the seed was measured by hardness tester. The Pearson correlation coefficient between the three indexes and pomegranate seed hardness was analyzed in order to discuss the influencing factors of pomegranate seed hardness and to provide references for the exploitation and utilization of pomegranate resources and the breeding of pomegranate seed. The results showed that there were some differences in kernel hardness, seed coat cellulose, lignin content and seed coat thickness between different pomegranate cultivars. There was a positive correlation between grain hardness and seed coat cellulose, lignin content and seed coat thickness, respectively 0.936, 0.965 and 0.994, respectively. The correlation between seed coat thickness and hardness was the most significant, followed by lignin content and cellulose content. The results showed that the hardness of pomegranate seed was affected by many factors such as seed coat cellulose, lignin content and thickness, The thickness of the seed coat is the most important factor.