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本文旨在研究限制饮食磷摄入对实验动物肾脏病(免疫模型)的肾功能保护作用。方法用鼠肾小球基底膜提纯液,反复免疫兔并取得肾毒血清,将此种血清注射到鼠体内引起肾毒血清性肾炎。在注射的2周内血清肌酐浓度有短时的急增,到第4周末肾功能恢复接近正常时,随机分成两组。A组13只鼠继续给予正常含磷饮食(0.5%),B组11只改予低磷饮食(0.04%)。结果尿蛋白排泄量:两组均明显增高,无显著差异。肾功能和存活率:A组呈进行性肾功能衰竭,在死亡时或133天实验结束时血清肌酐浓度为30±0.5mg/dl;而B组肾功能保持近于正常,在实验结束时肌酐浓度为0.93±0.2mg/dl(P<0.001)。B组存活(8/11)显著高于A组(1/13)(P<0.001)。A组全部死于肾功能衰竭,B组死亡3只中仅2只有血清肌酐升高。组织学改变:A组动物在光镜下有严
This article aims to investigate the effects of dietary phosphorus uptake on renal function in experimental animal models of nephropathy (immune model). Methods Rat glomerular basement membrane was purified by repeated immunization of rabbits and nephrotoxic serum was obtained. The serum was injected into mice to induce nephrotoxic serum nephritis. Serum creatinine concentrations increased rapidly in the shortest period of 2 weeks after injection. When the renal function recovered nearly normal at the end of the 4th week, they were randomly divided into two groups. Thirteen rats in group A continued to receive normal phosphorus diet (0.5%), while those in group B were switched to low phosphorus diet (0.04%). Results Urine protein excretion: Both groups were significantly higher, no significant difference. Renal function and survival rate: Group A was progressive renal failure, serum creatinine concentration was 30 ± 0.5 mg / dl at the time of death or at the end of 133 days of experiment, while the function of renal function in group B was close to normal. At the end of the experiment, creatinine The concentration was 0.93 ± 0.2 mg / dl (P <0.001). The survival in group B (8/11) was significantly higher than that in group A (1/13) (P <0.001). All of the A patients died of renal failure, and only two of the three deaths in the B group had elevated serum creatinine. Histological changes: Group A animals under light microscope