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一株 PSA单抗用于固相包被 ,另一株单抗与辣根过氧化物酶偶联制备抗 PSA酶标记物 ,以四甲基联苯胺 (TMB)为底物 ,采用二步法 ,建立了 PSA酶联免疫分析试剂盒 (EL ISA)制备方法。本方法的灵敏度为 0 .2 μg/ L,批内变异系数 <5.3% ,批间变异系数 <6 .4 % ,回收率为 96 .6 %～10 5.5% ,特异性鉴定显示与其他肿瘤标志物无明显交叉反应。正常参考值 <4 .0 μg/ L。本法所制试剂盒与 PSA- EL ISA试剂盒 (Maxim Biotech,Inc.)对照 ,两者相关方程为 y=0 .993x- 0 .16 ,相关系数 r=0 .932 (n=4 8)。该方法具有简便、快速和准确的特点 ,适于临床检测和科研应用。
One PSA monoclonal antibody was used for solid phase coating. The other monoclonal antibody was coupled with horseradish peroxidase to prepare anti-PSA enzyme markers. Using tetramethylbenzidine (TMB) as substrate, , Established PSA enzyme-linked immunosorbent assay kit (EL ISA) preparation. The sensitivity of this method was 0.2 μg / L, the intra-assay CV <5.3%, the inter-assay CV <6.4% and the recovery 96.6% -10.5%. The specificity of the assay was correlated with other tumor markers No obvious cross reaction. Normal reference <4 .0 μg / L The correlation coefficient r = 0.932 (n = 48), the correlation coefficient r = 0.993 (n = 48) . The method is simple, rapid and accurate, suitable for clinical testing and scientific research.