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目的 :探讨血源性脑及脑膜转移瘤中VEGF的表达水平及MRI检测意义。材料和方法 :38只新西兰大白兔随机分为三组 ,分别从颈总动脉行血脑屏障开放接种VX2癌细胞 ,单纯接种VX2癌细胞及单纯注射生理盐水。接种后1周开始在不同时间行MRI检查。病理取材HE染色光镜下观察 ,免疫组化方法检测脑及脑膜转移瘤中的VEGF表达水平。结果 :实验组脑膜成瘤率72 % ,脑内成瘤率62 %。MRI表现为T1WI等信号 ,T2WI低或高信号。注射Gd -TDPA后T1WI有明显的增强效应。VEGF检测证实血源性脑及脑膜转移瘤明显高于正常组。结论 :VEGF对脑及脑膜转移瘤的形成可能起重要作用 ,进一步研究VEGF抗体及其拮抗剂阻止或抑制脑及脑膜转移瘤是肿瘤治疗的发展方向。
Objective: To investigate the expression of VEGF in hematogenous brain and meningioma and the significance of MRI. MATERIALS AND METHODS: Thirty-eight New Zealand rabbits were randomly divided into three groups: VX2 cancer cells were inoculated from the common carotid artery to the blood-brain barrier, VX2 cells were inoculated alone, and saline alone was injected. One week after inoculation, MRI examination was performed at different times. Pathological specimens were observed under light microscope HE staining, immunohistochemistry detection of brain and brain metastases in VEGF expression levels. Results: The rate of tumor formation in the experimental group was 72% and the rate of tumor formation in the brain was 62%. MRI showed T1WI and other signals, T2WI low or high signal. After injection of Gd-TDPA T1WI significantly enhanced effect. VEGF test confirmed blood-derived brain and meningeal metastasis was significantly higher than the normal group. Conclusion: VEGF may play an important role in the formation of brain and meningeal metastases. To further study VEGF antibody and its antagonist to prevent or inhibit brain and meningioma metastasis is the development of cancer treatment.