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SRAP标记(sequence-relatedamplifiedpolymorphism,序列相关扩增多态性)是由Li和Quiros发展了一种新的分子标记技术。SRAP标记具有简便、稳定、中等产率、在基因组中分布均匀的特点,已被广泛利用。本实验利用甘薯高淀粉品种绵粉一号与甘薯低淀粉品种红旗四号杂交F1代分离群体,根据淀粉含量,选择F1代分离群体中高、低淀粉含量极端类型材料各23个构成选择性基因型子群体。构建的连锁图包括21个SRAP标记和9个连锁群(母本4个连锁群,父本5个连锁群)。复合作图检测到1个淀粉含量QTL,红旗4号的加性效应增加淀粉6.37%,解释表型变异的20.1%。
SRAP (sequence-related amplified polymorphism) is a new molecular marker technology developed by Li and Quiros. SRAP markers have been widely used because of their simplicity, stability, medium yield and even distribution in the genome. In this experiment, F1 hybrids of sweet potato high-starch variety F1 and sweet potato low-starch variety Hongqi No.4 were segregated. According to the starch content, 23 high-and low-starch content type of F1 segregation population were selected to form the selective genotypes Subgroups. The constructed linkage map consisted of 21 SRAP markers and 9 linkage groups (4 linkage groups and 5 linkage groups). One QTL for starch content was detected by composite mapping. The additive effect of Hongqi No.4 increased starch by 6.37%, accounting for 20.1% of phenotypic variation.