论文部分内容阅读
目的通过对广西艾滋病病毒Ⅰ型(HIV-1)新近感染者耐药基因变异的研究,了解当地HIV耐药毒株传播的水平。方法通过序贯抽样的方法,采集70例2007年8-10月新确证的、年龄在15～25岁的HIV感染者的全血样本,使用In-house法扩增HIV-1 pol区部分基因片段,并进行耐药基因变异分析。结果得到47条序列,其中有1份在蛋白酶区发生主要耐药突变M46L,可引起对ATV和NFV的低水平耐药性;在反转录酶区存在T69NT突变,仅引起对d4T和DDI的潜在耐药性。根据WHO HIV耐药警戒线监测方法判断,广西HIV耐药株属于低传播水平(<5%)。结论广西HIV新近感染者中已有HIV耐药株的传播,但是目前传播水平还较低,应继续开展HIV耐药毒株传播的监测工作。
OBJECTIVE: To study the variation of drug resistance genes in newly infected HIV-1 patients in Guangxi and to understand the transmission level of HIV-resistant strains in local areas. Methods A total of 70 whole blood samples of HIV-infected individuals aged 15-25 years old from August 2007 to October 2007 were collected by sequential sampling method. Some genes of HIV-1 pol region were amplified by In-house method Fragment, and analysis of resistance gene mutation. The results obtained 47 sequences, of which 1 in the protease region of the major drug-resistant mutation M46L, can cause low levels of resistance to ATV and NFV; presence of reverse transcriptase T69NT mutation, only cause d4T and DDI Potential drug resistance. According to WHO HIV resistance surveillance line monitoring method, Guangxi HIV-resistant strains belong to the low level of transmission (<5%). Conclusion The transmission of HIV-resistant strains among newly-infected HIV patients in Guangxi has been reported. However, the current level of transmission is still low. Monitoring of transmission of HIV-resistant strains should be continued.