论文部分内容阅读
目的 优化并评估一种新的探索基因组单核苷酸多态 (single nucleotide polymorphism,SNP)的温度调控高效液相色谱技术。方法 从洗脱方式、洗脱温度、检测器流通池规格、适应片段大小、检测灵敏度等方面 ,探讨适合探索基因组 SNP的温度调控高效液相色谱技术参数及实用价值。结果 找到在普通高效液相色谱仪上检测 SNP的具有普遍意义的色谱条件。 d NTP、PCR引物峰与被检测 PCR产物峰完全分离 ,纯合子和杂合子样本能有效区分。建立起在普通高效液相色谱仪上进行快速筛选突变型DNA的温度调控高效液相色谱法。结论 温度调控高效液相色谱法是一种高效、灵敏、操作简便、对较大片段具有普遍适用意义的探索基因组 SNP方法
OBJECTIVE: To optimize and evaluate a novel temperature-controlled high performance liquid chromatography (HPLC) technique to explore genomic single nucleotide polymorphisms (SNPs). Methods The parameters and practical value of temperature-controlled high performance liquid chromatography suitable for exploring genomic SNPs were explored from the aspects of elution method, elution temperature, detector flow cell size, size of adaptable fragments and detection sensitivity. Results Find common chromatographic conditions for detecting SNPs on a common HPLC. d NTP, the PCR primer peak completely separated from the detected PCR product peak, homozygous and heterozygous samples can be effectively distinguished. A temperature-controlled high performance liquid chromatography (HPLC) method was established for the rapid screening of mutant DNA on a common HPLC. Conclusion Temperature-controlled high performance liquid chromatography is an efficient, sensitive and easy-to-use method for genomic SNP detection