论文部分内容阅读
目的研究氯胺酮在大白兔体内死后弥散过程和再分布机制。方法 48只实验大白兔随机分为8组,采用缺氧处死后以150mg/kg氯胺酮灌胃,尸体仰卧位于室温下放置;在0~96h内分8个时间点各解剖1组,提取体液和脏器组织样品;采用GC/MS法定性结合GC-NPD法定量检测样品中氯胺酮含量,并计算心血/外周血中氯胺酮含量的比值。结果大白兔死后氯胺酮灌胃尸体放置96h内,脑、尿液、玻璃体液、左上/下肢肌肉样本中均未检测到氯胺酮,心血、外周血、心肌、脾、肾、肝、肺、胆汁中氯胺酮含量随死后时间呈动态升高的变化;其中距离胃较近的组织(如脾)较早检测到含量较高的氯胺酮,而距离较远的组织或体液中氯胺酮含量较低且较晚检测到;心血/外周血中氯胺酮含量比值为1.73。结论氯胺酮在家兔体内存在死后再分布,从胃到器官组织、心血顺浓度梯度弥散是主要机制。脑、玻璃体液、尿液、肢体肌肉不受死后弥散的影响,可作为生前服毒与死后染毒氯胺酮的鉴别依据。
Objective To study the mechanism of post-mortem dispersion and redistribution of ketamine in rabbits. Methods Forty-eight rabbits were randomly divided into 8 groups. After hypoxia, the rabbits were gavaged with ketamine at a dosage of 150 mg / kg. The body was placed in supine position at room temperature. At 0-86 h, Tissue sample was determined by GC / MS. The content of ketamine was quantitatively determined by GC / MS and GC-NPD. The ratio of ketamine in blood was calculated. Results Ketamine was not detected in brain, urine, vitreous humor, left upper / lower limb muscle samples within 96 h after ketamine instilled into corpses of rabbits after death Ketamine levels increased dynamically with post-mortem time; ketamine with higher content was detected earlier in tissues from the stomach (eg, the spleen), while ketamine levels were lower and later in tissues or fluids at longer distances The ratio of ketamine in cardiovascular / peripheral blood was 1.73. Conclusions Ketamine is redistributed in rabbits after death, and the main mechanism is gradient diffusion of blood concentration from stomach to organ tissue. Brain, vitreous humor, urine, limb muscles are not affected by the dispersion after death can be used as a prenatal drug poisoning and post-mortem ketamine identification based on.