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自DNA检验应用于法医鉴定以来,经过了十几年的发展,已建立了许多各种各样的方法和技术.STR分析方法虽发展时间不长,但由于其本身特有的特点,如等位基因片段短小,易于扩增,灵敏度高;基因座多,识别力强;对降解DNA能进行分析,可检验腐败陈旧生物检材;易于实现自动分析和计算机管理等,已日益成为日常DNA分析的主要、首选方法.STR分析从单个位点扩增分析到多个位点复合扩增分析,从银染到荧光检测分析,日趋自动化.反过来,检验程序的自动化进一步促进STR技术在日常案件检验中的应用.现荧光标记检测STR复合基因座技术已开始在法医DNA检验中应用并将被广泛采用.本文就其应用中的一些问题进行讨论.
Since the application of DNA test in forensic medicine, many methods and techniques have been established since the development of DNA testing for more than ten years.STR analysis has not been developed for a long time, but due to its unique characteristics such as allele Gene fragments are short, easy to amplify, high sensitivity; generous locus, strong recognition; degradation of DNA can be analyzed to detect spoiled stale biological samples; easy to implement automatic analysis and computer management, has increasingly become a daily DNA analysis Major, Preferred Methods .STR Analysis Increasing automation from single-site amplification assays to multiple site multiplex amplification assays, from silver to fluorescence assays, and conversely, automating test procedures further enhances STR’s ability to routinely test The application of fluorescently labeled STR complex locus technology has been started to be applied in forensic DNA testing and will be widely used.This paper discusses some of its applications.