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目的通过观察丙烯酰胺对大鼠神经行为的影响,建立神经行为功能观察组合(Functional Observational Battery,FOB)方法。方法将SD大鼠按体重随机分成3组,分别为溶媒对照组,丙烯酰胺20和50 mg/kg剂量组。每组20只,雌雄各半。每天1次,给药10 d后进行功能观察组合测试。测试方法包括笼内观察、手持观察、开场观察、操作性测试和生理指标测量。各指标代表神经系统不同功能区域,各数据资料都转换成严重性得分形式的等级资料,各功能区单项指标得分相加,进行单因素方差统计分析。结果丙烯酰胺在神经肌肉功能、自发性活动和神经兴奋性等方面对大鼠都产生了较明显的神经毒性,神经肌肉功能严重性得分:20 mg/kg丙烯酰胺组雄鼠10.50±2.27(P≤0.001)、雌鼠10.67±2.24(P≤0.01),50 mg/kg丙烯酰胺组雄鼠23.00±1.41(P≤0.001);雌鼠22.44±1.51(P≤0.001)。结论在GLP实验室建立的大鼠神经行为功能观察组合方法,指标全面,可以用于化学品与药品的神经毒性的初级评价。
Objective To observe the effect of acrylamide on neurobehavioral behavior of rats and establish a functional observational battery (FOB) method. Methods SD rats were randomly divided into three groups according to body weight: vehicle control group, acrylamide 20 and 50 mg / kg. Each group of 20, male and female half. Once a day, 10 days after administration of functional observation combined test. Test methods include in-cage observation, hand-held observation, opening observation, operational tests and physiological measurements. Each index represents the different functional areas of the nervous system, and all the data are converted into grading data in the form of severity scores. The single indexes of each functional area are added together and the single-factor variance statistical analysis is performed. Results Acrylamide had obvious neurotoxicity on neuromuscular function, spontaneous activity and excitability of nerve. The severity of neuromuscular function scores: 10.50 ± 2.27 (P ≤0.001), females10.67 ± 2.24 (P≤0.01), acrylamide males50.00 ± 1.41 (P≤0.001) and females22.44 ± 1.51 (P≤0.001). Conclusion The combination of neurobehavioral observation in rats established in GLP laboratory has comprehensive indexes and can be used for primary evaluation of neurotoxicity of chemicals and drugs.