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目的对天女木兰叶的化学成分进行分离和结构鉴定。方法采用硅胶、凝胶柱色谱和重结晶等分离方法对天女木兰叶的体积分数为90%的乙醇溶液提取物进行成分分离;通过谱学分析方法结合化合物理化性质对分离得到的化合物进行结构鉴定。结果分离得到8个化合物,分别鉴定为豆甾-4-烯-3,6-二酮(stigmast-4-en-3,6-dione,1),豆甾-4-烯-3β,6β-二醇(stigmast-4-en-3β,6β-diol,2),5α,8α-过氧-(22E,24R)-麦角甾-6,22-二烯-3β-醇[5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol,3],豆甾-4-烯-6β-羟基-3-酮(stigmast-4-en-6β-ol-3-one,4),β-谷甾醇(β-sitosterol,5),(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇[(22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol,6],豆甾-5-烯-3β,7α-二醇(stigmast-5-en-3β,7α-diol,7),胡萝卜苷(daucosterol,8)。结论化合物2-4、6、7为首次从木兰属植物中分离得到,化合物1为首次从该植物中分离得到。
Objective To isolate and identify the chemical constituents of Magnolia officinalis leaves. Methods Silica gel, gel column chromatography and recrystallization were used to separate the constituents of 90% ethanol extract of Magnolia sieboldii. The structures of the isolated compounds were identified by spectroscopic analysis and physical and chemical properties . Results Eight compounds were isolated and identified as stigmast-4-en-3,6-dione (1), stigmast-4-ene-3β, 6β- (Stigmast-4-en-3β, 6β-diol, 2), 5α, 8α-peroxo- (22E, 24R) -ergosta-6,22-diene-3β- - (22E, 24R) -ergosta-6,22-dien-3β-ol, 3], stigmast-4-en-6β-ol-3-one , 4), β-sitosterol (5), (22E, 24R) -ergosta-7,22-diene-3β, 5α, 6β-triol [(22E, 24R) -ergosta-7 , 22-dien-3β, 5α, 6β-triol, 6], stigmast-5-en-3β, 7α- diol (7), daucosterol 8). Conclusion Compounds 2-4, 6 and 7 were isolated from Magnoliaceae for the first time. Compound 1 was isolated from this plant for the first time.