本实验采用免疫电镜负染、细胞超薄切片透射电镜观察 ,证实所分离的病毒为犬呼肠病毒。病毒粒子由致密的核心和包围着核心的双层衣壳组成 ,有的为空心衣壳 ,多呈晶格状排列 ;病毒在胞浆增殖 ,装配 ,通过细胞崩解释放 ;细胞浆内可见大量的微丝与微管结构 ,认为与病毒复制有关。胞浆与胞核中均可见到内容物不同 ,形态各异的包涵体 In this experiment, negative staining by immunoelectron microscopy, transmission electron microscopy observation of ultrathin cells, confirmed that the isolated virus is canine reovirus. Virus particles by the dense core and surrounded by the core of the double capsid composition, and some hollow capsid, mostly lattice arrangement; virus in the cytoplasm proliferation, assembly, release by cell disintegration; cytoplasm can be seen in large quantities Microfilament and microtubule structure, that is related to the virus replication. Cytoplasm and nucleus can be seen in different contents, different forms of inclusion body