为探讨溴氰菊酯 (Deltamethrin ,DM )神经毒作用机理 ,采用竞争性固相酶联免疫法 (ELISA)和放射性同位素法分别观察了DM对不同脑区钙调蛋白 (Calmodulin ,CaM )含量和蛋白激酶 (Proteinkinase)活性的影响。结果表明 ,大鼠经DM处理后 ,大脑和小脑CaM含量无明显变化 ,而大脑皮层和海马中PKA ,PKC活性明显增强 ,同时 ,脑细胞膜和细胞浆中PKC活性亦明显升高。说明蛋白激酶活性增高使脑内蛋白质磷酸化作用增强可能是DM神经毒作用机理之一。 In order to explore the mechanism of neurotoxicity of Deltamethrin (DM), the contents of Calmodulin (CaM) in different brain regions were measured by competitive enzyme-linked immunosorbent assay (ELISA) and radioisotope method Proteinkinase activity. The results showed that the content of CaM in brain and cerebellum did not change significantly after DM treatment in rats, while the activity of PKA and PKC in cerebral cortex and hippocampus increased obviously. Meanwhile, the PKC activity in brain cell membrane and cytoplasm increased obviously. It indicates that the increase of protein kinase activity makes the increase of protein phosphorylation in the brain possibly one of the mechanisms of DM neurotoxicity.