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目的了解内蒙古地区结核分枝杆菌临床分离株的串联重复序列(variable number tandem repeat,VNTR)基因多态性和VNTR基因型构成,及不同VNTR位点在该地区结核分枝杆菌基因分型中的应用。方法从内蒙古自治区结核病防治研究所收集临床分离的结核分枝杆菌菌株及其病例背景资料,采用多位点串联重复序列分析(MLVA)对收集的菌株进行22个VNTR位点分析,计算Hunter-Gaston指数(HGDI),分析各个位点的分辨率,同时用BioNumerics软件对VNTR结果进行聚类分析。且统计学分析民族与主要基因型间的关系。结果 372株菌共分为308个基因型,47簇,261个独特基因型,成簇率为17.20%。22个VNTR位点的基因多态性存在较大的差异,位点VNTR3820(HGDI 0.838)的分型分辨能力最高,MIRU23(HGDI 0.068)和MIRU27(HGDI 0.083)分型分辨能力较差。随着VNTR位点的增加,分型的分辨能力也有所提高。Ⅰ群基因型菌株与民族易感性的分析表明,汉族与蒙古族间Ⅰ群基因型菌株分布差异无统计学意义(χ2=0.337,P=0.561>0.05)。结论内蒙古地区结核分枝杆菌临床分离株基因具有多态性,不同VNTR位点在内蒙古地区结核分枝杆菌中具有不同的分辨能力。且Ⅰ群基因型为该地区主要流行株,而Ⅰ群基因型菌株与民族易感性间无关联。
Objective To understand the polymorphism of VNTR gene and the genotype of VNTR in clinical isolates of Mycobacterium tuberculosis from Inner Mongolia and the genotypes of VNTR in different regions of Mycobacterium tuberculosis application. Methods The clinical isolates of Mycobacterium tuberculosis were collected from the Institute of Tuberculosis Prevention and Control of Inner Mongolia Autonomous Region and their case background data were collected and analyzed by MLVA for 22 VNTR loci. The Hunter-Gaston Index (HGDI) was used to analyze the resolution of each loci. At the same time, cluster analysis of VNTR results was performed with BioNumerics software. And statistically analyzed the relationship between ethnic groups and major genotypes. Results 372 strains were divided into 308 genotypes, 47 clusters and 261 unique genotypes. The clustering rate was 17.20%. There were significant differences in the polymorphism of 22 VNTR loci. The locus VNTR3820 (HGDI 0.838) had the highest genotyping ability, but the MIRU23 (HGDI 0.068) and MIRU27 (HGDI 0.083) were less well differentiated. With the increase of VNTR loci, the resolution of typing has also been improved. The analysis of the genotypes and ethnic susceptibility of group Ⅰ showed that there was no significant difference in genotype distribution between the Han and Mongolian groups (χ2 = 0.337, P = 0.561> 0.05). Conclusion The clinical isolates of Mycobacterium tuberculosis in Inner Mongolia are polymorphic. Different VNTR loci have different resolving power in Mycobacterium tuberculosis in Inner Mongolia. The genotypes of group Ⅰ were the main epidemic strains in this area, while the genotypes of group Ⅰ were not associated with ethnic susceptibility.