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[目的]探讨肠易激综合征(IBS)患者结肠黏膜降钙素基因相肽(CGRP)和P物质(SP)表达水平及与IBS临床症状的相关性。[方法]对腹泻型IBS(IBS-D组)20例、便秘型IBS(IBS-C组)8例和正常对照组5例各取乙状结肠黏膜标本,应用免疫组织化学染色法分别检测CGRP和SP;同时以评分法评价消化道症状;分析各组CGRP、SP表达水平与消化道症状的相关性。[结果]IBS-D组CGRP表达水平显著高于IBS-C组、正常对照组(0.27±0.08∶0.21±0.06、0.19±0.04,P<0.05),IBS-C组与正常对照组比较差异无统计学意义。IBS-D组SP表达水平显著高于IBS-C组、正常对照组(0.27±0.11∶0.19±0.04、0.17±0.04,P<0.05),IBS-C组与正常对照组比较差异无统计学意义。肠黏膜SP表达水平与IBS患者腹痛(r=0.495,P=0.007)、排便变稀(r=0.382,P=0.045)呈正相关,与排便困难(r=-0.485,P=0.009)、排便干结(r=-0.382,P=0.045)负相关;CGRP与排便变稀(r=0.401,P=0.034)正相关,与排便干结(r=-0.401,P=0.034)负相关。[结论]CGRP、SP可能参与IBS的病理生理过程并与临床症状密切相关。
[Objective] To investigate the correlation between the expression of calcitonin gene phase peptide (CGRP) and substance P (SP) in patients with irritable bowel syndrome (IBS) and the clinical symptoms of IBS. [Methods] 20 cases of IBS (IBS-D group), 8 cases of constipation-type IBS (IBS-C group) and 5 cases of normal control group were divided into four groups. Immunohistochemistry was used to detect the expression of CGRP and SP At the same time, the gastrointestinal symptoms were evaluated by the score method. The correlation between CGRP and SP expression and gastrointestinal symptoms was analyzed. [Results] The expression of CGRP in IBS-D group was significantly higher than that in IBS-C group, normal control group (0.27 ± 0.08:0.21 ± 0.06,0.19 ± 0.04, P <0.05). There was no difference between IBS-C group and normal control group Statistical significance. The expression of SP in IBS-D group was significantly higher than that in IBS-C group and normal control group (0.27 ± 0.11:0.19 ± 0.04, 0.17 ± 0.04, P <0.05). There was no significant difference between IBS-C group and normal control group . The expression of SP in intestinal mucosa was positively correlated with abdominal pain (r = 0.495, P = 0.007), thinning of stool (r = 0.382, P = 0.045) and difficulty in defecation (r = -0.485, P = 0.009) (r = -0.382, P = 0.045). CGRP was negatively correlated with feces excretion (r = 0.401, P = 0.034) and negative correlation with defecation (r = -0.401, P = 0.034) [Conclusion] CGRP and SP may participate in the pathophysiological process of IBS and are closely related to clinical symptoms.