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[目的]观察白陶土联合普萘洛尔对百草枯(PQ)中毒小鼠肺组织毒物浓度的影响。[方法]144只ICR小鼠随机分为3组:PQ组、PQ+白陶土联合普萘洛尔组(下称治疗组)、对照组,PQ组、治疗组予PQ 100 mg/kg灌胃,治疗组在PQ灌胃后立即予白陶土48 g/kg联合普萘洛尔3.2 mg/kg灌胃,对照组给予等体积生理盐水灌胃,每组在造模后0.5、1、2、4、6、8、12、24 h时间点取小鼠肺组织进行病理检查,制作匀浆进行PQ浓度检测。[结果]PQ组小鼠肺组织出现肺泡毛细血管扩张,内皮细胞肿胀,小灶状或大片状炎性细胞浸润,以中性粒细胞为主,治疗组上述病变程度明显减轻。PQ组和治疗组小鼠灌胃0.5 h后肺组织PQ浓度明显上升,4 h达高峰;与PQ组相比,治疗组第4 h开始肺组织PQ浓度明显降低(P<0.05),持续至24 h。[结论]白陶土联合普萘洛尔可以降低PQ中毒小鼠肺组织毒物浓度,减轻肺部损伤,联合使用值得在临床上进一步研究。
[Objective] To observe the effect of kaolin combined with propranolol on lung tissue toxic concentration of paraquat (PQ) poisoned mice. [Methods] 144 ICR mice were randomly divided into three groups: PQ group, PQ + kaolin combined with propranolol group (hereinafter referred to as treatment group), control group, PQ group, treatment group given PQ 100 mg / In the treatment group, kaolin 48 g / kg and propranolol 3.2 mg / kg were intragastrically administered immediately after the intragastric administration of PQ, and the control group was given the same volume of normal saline. Each group was given 0.5, 1, 2, 4 At 6, 8, 12 and 24 h, the lung tissues of mice were taken for pathological examination, and the homogenates were prepared for the detection of PQ concentration. [Results] Pulmonary tissue of mice in PQ group showed alveolar telangiectasia, swollen endothelial cells, small focal or large inflammatory cells infiltration, mainly neutrophils, the treatment group significantly reduced the degree of disease. Compared with PQ group, the PQ concentration in lung tissue of PQ group and treatment group increased significantly (P <0.05), and peaked at 4 h 24 h. [Conclusion] The combination of kaolin and propranolol can reduce the concentration of pulmonary tissue poisoning in mice with PQ poisoning and relieve lung injury. The combination of kaolin and propranolol should be further studied clinically.