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目的 :探讨转化生长因子 β1(TGF β1)与喉鳞状细胞癌患者的病理分级、临床分期间的相互关系 ,研究TGF β1在喉鳞状细胞癌发病机制中的作用。方法 :分别提取 2 1例喉鳞状细胞癌组织、15例喉正常组织的总RNA ,采用逆转录聚合酶链反应技术 ,检测TGF β1mRNA的水平。结果 :2 1例喉鳞状细胞癌组织中 10例(47.6 2 %)TGF β1mRNA表达阳性。TGF β1mRNA与喉鳞状细胞癌病理分级和临床分期有一定关系。结论 :TGF β1可作为评定喉鳞状细胞癌的进展、浸润的指标。
Objective: To investigate the relationship between TGFβ1 and laryngeal squamous cell carcinoma in pathological grade and clinical stage, and to explore the role of TGFβ1 in the pathogenesis of laryngeal squamous cell carcinoma. Methods: Total RNA was extracted from 21 laryngeal squamous cell carcinoma tissues and 15 normal laryngeal tissues respectively. The mRNA level of TGF β1 was detected by reverse transcription polymerase chain reaction (RT-PCR). Results: The expression of TGFβ1 mRNA in 10 of 21 (47.6%) laryngeal squamous cell carcinomas was positive. TGF β1 mRNA and laryngeal squamous cell carcinoma of the pathological grade and clinical stage have a certain relationship. Conclusion: TGFβ1 can be used as an index to evaluate the progress and infiltration of laryngeal squamous cell carcinoma.