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目的探讨腺病毒介导的胞嘧啶脱氨酶基因对小鼠肝癌的基因治疗效果。方法分离纯化AdCD，体外实验观察IC_(50)，旁观者效应及凋亡现象。在体内，AdCD瘤内注射并腹腔注射5FC，观察AdCD／5FC系统对肝癌荷瘤小鼠的抗肿瘤效应。结果当MOI＝100时，AdCD感染的MM45T.Li细胞对5FC敏感,IC_(50)＜50μmol／L。与AdCD感染的MM45T.Li细胞占10％时，可有72％细胞死亡,AdCD／5FC系统对MM45T.Li细胞有明显的旁杀伤效应。TUNEL检测及Hoechst33258荧光染色证实AdCD／5FC系统可以诱导部分MM45T.Li细胞凋亡。荷瘤小鼠经过治疗21d后，各对照组肿瘤体积均数为2303～2943mm~3，治疗组AdCD／5FC组肿瘤体积（365±81）mm~3，ASdCD／5FC系统治疗小鼠肝癌，可以明显减小肿瘤体积（F＝20.33，P＜0.05）.对照组中位生存期41～43d,治疗组中位生存期49d。结论AdCD／5FC系统对小鼠肝癌有明显的治疗作用。
Objective To investigate the gene therapy effect of adenovirus-mediated cytosine deaminase gene on mouse liver cancer. Methods AdCD was isolated and purified. IC 50, bystander effect and apoptosis were observed in vitro. In vivo, AdFCs were injected intraperitoneally and intraperitoneally injected with 5FC to observe the antitumor effect of AdCD / 5FC system on hepatocellular carcinoma-bearing mice. Results When MOI = 100, Ad-infected MM45T.Li cells were sensitive to 5FC with IC 50 (50μmol / L). 72% of cells died when MM45T.Li cells infected with AdCD accounted for 10%, and AdCD / 5FC system had obvious para-lethal effect on MM45T.Li cells. TUNEL assay and Hoechst33258 fluorescence staining confirmed that AdCD / 5FC system can induce apoptosis of some MM45T.Li cells. The tumor volume of each control group was 2303 ~ 2943mm ~ 3 after 21 days of treatment, the tumor volume was (365 ± 81) mm ~ 3 in AdCD / 5FC group and the treatment of mouse liver cancer by ASdCD / 5FC system (F = 20.33, P <0.05), the median survival time was 41-43 days in the control group, and the median survival time in the treatment group was 49 days. Conclusion AdCD / 5FC system has obvious therapeutic effect on mouse liver cancer.