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实验用日本白色家兔,分别以10、20、70、250及800条日本血吸虫尾蚴经皮肤接种。感染一周后,从兔耳静脉取血,分离血清,保存于-20℃。用2囝吡喹酮悬液给感染9周的家兔以100mg/kg分4次间隔3小时灌入胃内。抗原是冰冻干燥的日本血吸虫虫卵及成虫用炭酸盐缓冲液提取的。酶联免疫吸附试验:被检血清用1%BSA-PBS-Tween从1:20作倍比稀释。以正常兔血清OD值的两倍以上作为抗体效
Experiments with Japanese white rabbits, respectively, 10,20,70,250 and 800 of Schistosoma japonicum cercariae by skin inoculation. One week after the infection, blood is drawn from the rabbit ear vein and the serum is separated and stored at -20 ° C. Rabbits infected for 9 weeks were infused with 2 ml of praziquantel suspension into the stomach at intervals of 3 hours at 100 mg / kg for 3 hours. Antigen is freeze-dried Schistosoma japonicum eggs and adults with carbonate buffer extraction. Enzyme-linked immunosorbent assay: Serum sera were diluted 1: 20 in 1% BSA-PBS-Tween. To normal rabbit serum OD value of more than twice as anti-efficacy