论文部分内容阅读
通过分析不同浓度Ca2+与SA对高温胁迫下对桔梗抗逆生理指标及相关光合参数的影响,了解Ca2+,SA对桔梗高温胁迫伤害调控的相关性,为缓解桔梗高温胁迫提供依据。该实验以盆栽桔梗为材料,叶面喷施不同浓度Ca Cl2,SA溶液,然后置于35℃/25℃(昼/夜),光照3 600 lx的光照培养箱内,进行高温胁迫处理,以常温25℃喷施蒸馏水和高温胁迫下喷施蒸馏水处理为对照,测定桔梗叶片相关光合参数,膜透性,脯氨酸,丙二醛,可溶性蛋白,ASA,GSH含量,以及SOD,CAT活性。结果表明,6 mmol·L-1Ca Cl2,1.5 mmol·L-1SA可显著增强桔梗叶片SOD,CAT酶活性,提高叶片游离脯氨酸及可溶性蛋白含量,有效减少高温胁迫对细胞膜的破坏,显著降低相对电导率,同时,处理提高了叶片中叶绿素与类胡萝卜素含量,提高了叶片光合效率,有效增加ASA,GSH的含量,从而抵御高温造成的氧化胁迫,但随着浓度的增大,桔梗叶面抵御高温胁迫的能力则会下降。喷施一定浓度Ca Cl2,SA溶液可减轻高温胁迫对桔梗叶片的伤害。
The effects of different concentrations of Ca2 + and SA on physiological response and photosynthetic parameters of Campanulaceae were studied under high temperature stress to understand the correlation between Ca2 + and SA on the regulation of Hippophae rhamnoides under high temperature stress and to provide basis for alleviating the high temperature stress of Campanulaceae. In this experiment, Platycodon grandiflorum was used as material, and different concentrations of CaCl 2 and SA solution were sprayed on the foliage, and then placed in a light incubator at 35 ℃ / 25 ℃ (day / night) and light exposure of 3 600 lx for high temperature stress treatment The photosynthesis parameters, membrane permeability, proline, malondialdehyde, soluble protein, ASA and GSH contents, and the activities of SOD and CAT in leaves of Campanulaceae were determined by spraying distilled water at 25 ℃ and distilled water under high temperature stress. The results showed that 6 mmol·L-1 CaCl 2 and 1.5 mmol·L-1 SA significantly increased SOD and CAT activities, increased free proline and soluble protein content in leaves, decreased the damage of cell membrane under high temperature stress and decreased significantly Relative conductivity, at the same time, treatment increased leaf chlorophyll and carotenoid content, increased leaf photosynthetic efficiency, increased ASA, GSH content, thereby protecting against oxidative stress caused by high temperature, but with increasing concentration, Surface resistance to high temperature stress will decline. Spraying a certain concentration of Ca Cl2, SA solution can reduce the damage to the leaf of Platycodon grandiflorum under high temperature stress.