①目的探讨阿霉素诱导Ｓ－１８０瘤细胞凋亡过程中细胞核型的变化。②方法用低剂量（３．２５μｇ）阿霉素处理经Ｓ－１８０瘤细胞腹腔接种的ＢＡＬＡ／Ｃ鼠，０～７２ｈ分别取腹水进行细胞核型检测。③结果用阿霉素处理２４ｈ时，细胞变圆，胞浆浓缩；４８ｈ时，染色质密集度增高；７２ｈ时，核仁裂解，核膜内陷，形成内涵物不外溢的凋亡小体。④结论阿霉素是良好的细胞凋亡诱导剂，凋亡期间细胞外形及染色质凝聚逐渐改变，直至形成凋亡小体。 Objective To investigate the changes of karyotype in the process of apoptosis induced by doxorubicin in S-180 tumor cells. 2 Methods BALA/C mice inoculated intraperitoneally with S-180 tumor cells were treated with low dose (3.25μg) doxorubicin. Ascites were taken from 0 to 72h for karyotype detection. 3 Results When treated with doxorubicin for 24 hours, the cells became round and the cytoplasm was concentrated; at 48 hours, the chromatin density increased; at 72 hours, the nucleolus was lysed and the nuclear membrane retracted, forming apoptotic bodies with no inclusions. 4 Conclusions Doxorubicin is a good inducer of apoptosis, and cell morphology and chromatin condensation gradually change during apoptosis until the formation of apoptotic bodies.