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目的:探讨富氧对缺氧大鼠心肌损伤的保护作用及机理。方法:雄性Wistar大鼠32只随机分为平原对照组、低氧组、富氧组,低氧组在模拟海拔5400m低压舱内24h,富氧组在模拟5400m的低压舱内12h,然后富氧(27.0%的氧混合气)12h,观察心肌琥珀酸脱氢酶(SDH)及超微结构的改变。结果:低氧组心肌细胞灶性变性坏死,肌丝溶解凝固,间质水肿液和纤维素样渗出。富氧组心肌无明显病变,仅滑面内质网轻度扩张。富氧组SDH活性明显高于低氧组。结论:低氧导致心肌SDH活性及超微结构的改变,富氧对心肌有保护作用。
Objective: To explore the protective effect and mechanism of oxygen enrichment on myocardial injury induced by hypoxia in rats. Methods: Thirty-two male Wistar rats were randomly divided into three groups: control group, hypoxia group, oxygen-enriched group and hypoxia group. After simulating altitude of 5400m in low pressure cabin for 24h and oxygen-rich group in simulated low pressure cabin of 5400m for 12h, (27.0% oxygen mixture) for 12h, the changes of myocardial succinate dehydrogenase (SDH) and ultrastructure were observed. Results: Hypoxic group of myocardial cell degeneration and necrosis, myofibrillar coagulation, interstitial edema fluid and cellulose-like exudation. Oxygen-enriched group myocardial no obvious change, only gliding endoplasmic reticulum mild expansion. SDH activity in oxygen-enriched group was significantly higher than that in hypoxia group. CONCLUSION: Hypoxia leads to myocardial SDH activity and ultrastructural changes, and oxygen enrichment has a protective effect on myocardium.