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目的了解湖南地区中南大学三所附属医院产CTX-M型超广谱β-内酰胺酶大肠埃希菌的检出率,并确定其基因型。方法收集2004年10月-2005年7月临床标本分离的ESBLs表型阳性大肠埃希菌52株,PCR法进一步进行CTX-M酶基因型的检测,PCR产物测序并通过blast分析确定其基因分型。结果经多重PCR扩增,48株携带CTX-M基因,检出率为92.3%。共检出四种基因型,即CTX-M-3、CTX-M-14、CTX-M-15和CTX-M-82。结论大肠埃希菌中CTX-M型超广谱酶相当普遍,主要为CTX-M-14、CTX-M-3、CTX-M-15;其中CTX-M-82为新的基因型,经美国Lahey临床医学中心专家确认并命名,GenBank登录号为DQ256091。
Objective To investigate the detection rate of CTX-M type extended-spectrum β-lactamase-producing Escherichia coli in three affiliated hospitals of Central South University in Hunan Province and determine its genotype. Methods 52 strains of ESBLs-positive Escherichia coli isolated from clinical specimens from October 2004 to July 2005 were collected. The genotypes of CTX-M were further detected by PCR. The PCR products were sequenced and analyzed by blast analysis type. The results of multiplex PCR amplification, 48 strains carrying CTX-M gene, the detection rate was 92.3%. Four genotypes were detected, namely CTX-M-3, CTX-M-14, CTX-M-15 and CTX-M-82. Conclusion CTX-M type ESBLs are quite common in Escherichia coli, mainly CTX-M-14, CTX-M-3 and CTX-M-15. CTX-M-82 is a new genotype The United States of America Lahey Clinical Medical Center identified and named experts, GenBank accession number DQ256091.