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放射免疫分析法(RIA)是放射性同位素示踪技术与免疫反应原理相结合的一种体外的超微量分析方法。其特点是灵敏度高、特异性强、精确度好、取样量少、操作简便,不会引起生物体的辐射损伤。这种方法能定量分析毫微克—微微克水平的微量物质。被测物质达二三百种之多,目前已成为生物学和医学研究中不可缺少的手段。 RIA的基本原理是:在一溶液系统中,一定量的放射性同位素标记的抗原(~*Ag)和待测的非标记抗原(Ag)对一定量特异抗体(Ab)的竞争性结合。Ag的量愈大,~*Ag被稀释的程度也愈大,从而~*Ag-Ab
Radioimmunoassay (RIA) is an in vitro ultra-trace analysis method combining radioactive isotope tracing technique and immunological reaction principle. It is characterized by high sensitivity, specificity, accuracy, sample volume, easy to operate, will not cause radiation damage to the organism. This method quantifies trace amounts of nanograms to pico grams. Up to two hundred or three hundred kinds of substances tested, has now become an indispensable means of biology and medical research. The basic principle of RIA is that in a solution system, a certain amount of radioisotope-labeled antigen (~ * Ag) and a non-labeled antigen (Ag) to be tested for competitive binding to a specific amount of specific antibody (Ab). The greater the amount of Ag, the greater the extent of ~ * Ag dilution, so ~ Ag-Ab