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为阐明紫苏[Perilla frutescens(L.)Britt.]响应镉胁迫的分子机制,应用营养液加镉法,采用蛋白质组学技术分析了紫苏叶片响应镉胁迫3周的蛋白质表达差异。结果表明,镉胁迫下紫苏叶片有25个蛋白发生差异表达,其中20个蛋白质得到LC-MS/MS鉴定:光合作用相关蛋白3个,能量代谢相关蛋白11个,胁迫相关蛋白1个,蛋白质代谢相关蛋白2个,基因表达相关蛋白1个,结构蛋白1个,生物合成与解毒相关蛋白1个。在浓度为2.0 mg·kg-1、5.0 mg·kg-1、10.0mg·kg-1镉胁迫下,紫苏叶片中ATP合成酶、丝氨酸羧肽酶、植物细胞色素P450均上调表达,Rubisco大亚基、核糖体蛋白S3和肌动蛋白表达均下调。光合系统Ⅱ稳定/装配因子HCF136及胁迫反应蛋白乙酰辅酶A硫酯酶在低浓度镉(2 mg·kg-1)处理下表达上调,在高浓度镉(5 mg·kg-1,10 mg·kg-1)处理下表达下调;磷酸核酮糖激酶/尿苷激酶家族蛋白在2 mg·kg-1和5 mg·kg-1镉处理时表达上调,10 mg·kg-1镉处理时不变;逆转录转座子蛋白在10 mg·kg-1镉处理时表达下调。可见,紫苏叶片通过增强能量代谢、降低光合作用、改变蛋白代谢与基因表达和提高解毒能力,增强了镉耐性。
To elucidate the molecular mechanism of perilla frutescens (L.) Britt. Responding to cadmium stress, the protein expression of perilla frutescens (L.) Britt. The results showed that 25 proteins were differentially expressed in perilla leaves under cadmium stress. Among them, 20 proteins were identified by LC-MS / MS: 3 photosynthesis-related proteins, 11 energy metabolism related proteins, 1 stress-related proteins, 1 protein Two metabolized proteins, one gene-related protein, one structural protein, and one biosynthesis and detoxification related protein. Under the stress of 2.0 mg · kg -1, 5.0 mg · kg -1 and 10.0 mg · kg -1, ATP synthase, serine carboxypeptidase and plant cytochrome P450 in the leaves of Perilla frutescens were all up-regulated, Subunit, ribosomal protein S3 and actin expression were down-regulated. The photosynthetic system Ⅱ stable / assembly factor HCF136 and the stress response protein acetyl-CoA thioesterase were up-regulated under the low concentration cadmium (2 mg · kg -1) treatment. High concentrations of cadmium (5 mg · kg -1, 10 mg · kg -1) kg-1). Phosphofructokinase / uridine kinase family protein was up-regulated when treated with 2 mg · kg-1 and 5 mg · kg-1 cadmium, while not treated with 10 mg · kg-1 cadmium The expression of retrotransposon protein was down-regulated when treated with 10 mg · kg-1 cadmium. Visible, perilla leaves through enhanced energy metabolism, reduce photosynthesis, change protein metabolism and gene expression and improve detoxification ability, enhanced cadmium tolerance.