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为了有效利用缫丝蚕蛹的功能性成分开发高附加值的降血糖药物,采用95%乙醇浸提蚕蛹活性物质,并依次选用石油醚、乙醚、乙酸乙酯、水饱和正丁醇对蚕蛹醇提物进行梯度极性溶剂萃取,获得不同萃取组分及水相组分。测定各相萃取组分对α-葡萄糖苷酶的体外抑制活性,结果显示:当石油醚、乙醚、乙酸乙酯、正丁醇萃取组分及水相组分的质量浓度为0.33mg/mL时,各相萃取组分对α-葡萄糖苷酶的体外抑制率分别为100%、58%、38.1%、36.93%、21.53%;各相萃取组分对α-葡萄糖苷酶的半数抑制浓度(IC50)分别为0.049、0.272、0.434、0.645、0.449 mg/mL。选择对α-葡萄糖苷酶抑制活性较强的石油醚相和乙醚相萃取组分,采用双倒数作图法检测其抑制作用类型均属于非竞争性抑制。
In order to effectively use the functional components of silkworm pupa to develop high value-added hypoglycemic drugs, the active substance of silkworm pupae was extracted with 95% ethanol and petroleum ether, ethyl ether, ethyl acetate and saturated n-butanol The material was subjected to gradient polar solvent extraction to obtain different extraction components and aqueous phase components. The results showed that when the mass concentration of petroleum ether, ethyl ether, ethyl acetate, n-butanol extract and water phase components was 0.33mg / mL The inhibitory rates of α-glucosidase in vitro were 100%, 58%, 38.1%, 36.93% and 21.53%, respectively. The half inhibitory concentration of α-glucosidase ) Were 0.049,0.272,0.434,0.645,0.449 mg / mL respectively. Select the extract of petroleum ether and ether with strong α-glucosidase inhibitory activity, and determine the type of inhibition by double reciprocal mapping, all belong to non-competitive inhibition.