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目的探讨囊胚序贯培养系统在体外受精-胚胎移植技术(IVF-ET)中的临床应用。方法进行了17个周期的囊胚序贯培养,将胚胎体外培养至第5-6天,达囊胚期后移植。结果共取卵250枚,受精率68%,囊胚形成率为52%,囊胚种植率为28%,获8例临床妊娠。结论序贯培养囊胚移植更适合卵子和胚胎的体外发育,对提高临床妊娠率,降低多胎率有良好的应用前景。
Objective To investigate the clinical application of in vitro fertilization-embryo transfer (IVF-ET) system of blastocyst sequential culture system. Methods Seventeen cycle blastocysts were cultured in sequence. The embryos were cultured in vitro until day 5-6 and reached the blastocyst stage. Results A total of 250 eggs were obtained. The fertilization rate was 68%, the blastocyst formation rate was 52%, the blastocyst implantation rate was 28%, and 8 clinical pregnancies were achieved. Conclusion Sequential culture of blastocyst transplantation is more suitable for in vitro development of ovum and embryo, which has a good prospect of application in improving clinical pregnancy rate and reducing multiple birth rate.