论文部分内容阅读
土壤微生物多样性反映土壤生态系统健康程度,对土壤养分循环发挥决定作用,并在很大程度上影响林地生产力。为研究人工林长期经营对土壤微生物多样性的影响,本研究以杨树人工林为研究对象,取连作林地根际和非根际土壤,采用MOBIO PowerSoil DNA Isolation kit试剂盒提取土壤中微生物基因组DNA,并进行16S rDNAV3区扩增。结果表明:6种杨树人工林土壤中均能提取出微生物基因组DNA,基因组DNA片段大于2 000 bp,且DNA带型清晰完整,无明显降解,为后续土壤细菌的PCR扩增提供了良好模板;6种土壤基因组DNA在电泳图中的亮度不一,其中Ⅰ代林根际土壤(B3)微生物DNA条带最亮,Ⅲ代林非根际土壤(FB18)微生物DNA条带亮度最弱,经检测,B3样品DNA含量约为6.9 ng.cm-3,而FB18仅有2.0 ng.cm-3左右;选用27F/1492R和F338-GC/R518两对引物,采用巢式PCR策略,成功扩增出杨树人工林土壤细菌16S rDNA V3区DNA,片段大小为220 bp左右。
Soil microbial diversity reflects the soil ecosystem health and plays a decisive role in soil nutrient cycling and largely affects forest productivity. In order to study the effect of long-term management of plantations on the diversity of soil microorganisms, Populus plantation was used as the research object. The soil samples were extracted from the rhizosphere and non-rhizosphere soil of the continuous cropping habitat by MOBIO PowerSoil DNA Isolation kit , And 16S rDNAV3 region amplification. The results showed that the genomic DNA was extracted from the soil of six poplar plantations, the genomic DNA fragments were more than 2 000 bp, and the DNA bands were clear and complete without any obvious degradation, which provided a good template for subsequent PCR amplification of soil bacteria The brightness of DNA in six kinds of soil genomic DNA was different in the electrophoretogram. Among them, the DNA bands of microorganism in rhizosphere soil of B3 generation were the brightest and the bands of DNA of FB18 in the generation III were the weakest, The DNA content of B3 sample was about 6.9 ng.cm-3, while that of FB18 was only about 2.0 ng.cm-3. Two pairs of primers, 27F / 1492R and F338-GC / R518, The DNA of 16S rDNA V3 region of soil bacteria in poplar plantation was increased, and the fragment size was about 220 bp.