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目的:建立鹿红颗粒中黄芪甲苷和羟基红花黄色素A的含量测定方法。方法:采用HPLC-ELSD测定黄芪甲苷含量,Kromasil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(34∶66),流速1.0 mL·min-1,柱温25℃,ELSD参数为蒸发区温度90℃,雾化区温度40℃,气流速度1.50 mL·min-1;采用HPLC-VWD测定羟基红花黄色素A含量,流动相甲醇-乙腈-0.2%磷酸水(35∶2∶63),流速1.0 mL·min-1,柱温30℃,检测波长403 nm。结果:黄芪甲苷、羟基红花黄色素A的线性范围分别为0.165 2~10.58,0.021~1.34μg,平均回收率依次为98.56%(RSD 1.99%),101.84%(RSD 2.54%),样品中质量分数分别为0.333,1.803 mg·g-1。结论:建立的含量测定方法简单易行、重复性良好,适用于鹿红颗粒的质量评价。
Objective: To establish a method for determination of Astragaloside Ⅳ and hydroxysafflor yellow A in dehonghong granules. Methods: The content of astragaloside was determined by HPLC-ELSD. The mobile phase consisted of acetonitrile-water (34:66), flow rate of 1.0 mL · min-1, column temperature of 25 ° C on a Kromasil C18 column (4.6 mm × 250 mm, The ELSD parameters were as follows: the temperature of the evaporation zone was 90 ℃, the temperature of the atomization zone was 40 ℃ and the flow velocity was 1.50 mL · min-1. The content of hydroxysafflor yellow A was determined by HPLC-VWD. The mobile phase was methanol-acetonitrile-0.2% phosphoric acid : 2: 63), the flow rate was 1.0 mL · min-1, the column temperature was 30 ℃ and the detection wavelength was 403 nm. Results: The linear ranges of astragaloside and hydroxysafflor yellow A were 0.165 2 ~ 10.58 and 0.021 ~ 1.34 μg, the average recoveries were 98.56% (RSD 1.99%) and 101.84% (RSD 2.54%), respectively The mass fractions were 0.333 and 1.803 mg · g-1, respectively. Conclusion: The established method for the determination of content is simple, reproducible and suitable for the quality evaluation of deer granules.