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目的 :探讨神经元特异性烯醇化酶 (NSE)、肿瘤坏死因子 -α(TNF -α)及脂质唾液酸 (LSA)在肺癌中的表达及其诊断价值。方法 :收集肺癌病人血清 78份 ,良性肺病患者血清 32份 ,正常对照血清 10 9份。采用酶联免疫吸附试验 (ELISA)和化学发光免疫分析法分别测定血清中的NSE、TNF -α及LSA。结果 :肺癌患者血清中的NSE、TNF -α、LSA分别为 19 78± 12 10ng/ml、135 6 4± 4 9 0 1pg/ml、1 0 6± 0 31mg/ml,明显高于良性肺病组 7 5 6± 3 4 1ng/ml、84 70± 2 4 89pg/ml、0 78± 0 18mg/ml及正常对照组 8 0 1± 2 81ng/ml、71 2 5± 13 5 0pg/ml、0 70± 0 13mg/ml(p <0 0 1)。对肺癌诊断的阳性率分别为 6 2 82 %、84 6 2 %、75 6 4 。联合检测阳性率达92 31%。结论 :血清NSE、TNF -α、LSA在肺癌病人中有较高的阳性率 ,联检阳性率更高 ,故NSE、TNF -α、LSA的联检是肺癌的非常有用的辅助诊断指标。 阳性标准为NSE >16 0ng/ml,TNF -α >10 0pg/ml,LSA >0 5 8mg/ml。
Objective: To investigate the expression and diagnostic value of neuron specific enolase (NSE), tumor necrosis factor-α (TNF-α) and lipid sialic acid (LSA) in lung cancer. Methods: Seventy-eight patients with lung cancer, 32 patients with benign lung disease and 109 healthy controls were enrolled in this study. Serum levels of NSE, TNF-α and LSA were measured by enzyme-linked immunosorbent assay (ELISA) and chemiluminescence immunoassay. Results: The serum levels of NSE, TNF-α and LSA in lung cancer patients were 19 78 ± 12 10 ng / ml, 135 6 4 ± 4 9 01 pg / ml, and 106 ± 0 31 mg / ml respectively, which were significantly higher than those in benign lung disease group 7 5 6 ± 3 4 1 ng / ml, 84 70 ± 2 4 89 pg / ml, 0 78 ± 0 18 mg / ml, and normal control 8 0 1 ± 2 81 ng / ml, 71 2 5 ± 135 0 pg / ml, 0 70 ± 0 13 mg / ml (p <0 0 1). The positive rates of diagnosis of lung cancer were 6282%, 8462% and 7564 respectively. Joint detection of positive rate of 92 31%. Conclusion: Serum NSE, TNF-α and LSA have a higher positive rate in lung cancer patients and a higher positive rate of joint detection. Therefore, the combination of NSE, TNF-α and LSA is a useful diagnostic indicator for lung cancer. The positive criteria were NSE> 16 0 ng / ml, TNF-a> 10 0 pg / ml and LSA> 0 58 mg / ml.