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目的:建立新疆鼠尾草以三萜为有效部位的熊果酸含量的高效液相色谱检测方法。方法:采用高效液相色谱法(HPLC法)检测浸膏中熊果酸的含量,色谱柱为Waters Symmetry C18柱(4.6mm×250mm,5μm),流动相为甲醇—水—冰乙酸—三乙胺(85:15:0.03:0.06):甲醇=70:30,流速0.8ml/min,检测波长210nm,柱温30℃。结果:HPLC法测得熊果酸的线性范围为Y=447684X-7680(r=0.9999),平均回收率为98.84%,RSD=1.69%(n=6)。结论:HPLC法测定熊果酸含量,操作简便,灵敏度高,重现性好,可为新疆鼠尾草以三萜为有效部位的质量控制提供一定的依据。
Objective: To establish a HPLC method for the determination of ursolic acid in Triterpenes. Methods: The content of ursolic acid in extracts was determined by high performance liquid chromatography (HPLC). The chromatographic column was a Waters Symmetry C18 column (4.6mm×250mm, 5μm). The mobile phase was methanol-water-glacial acetic acid-3 Amine (85:15:0.03:0.06):methanol=70:30, flow rate 0.8 ml/min, detection wavelength 210 nm, column temperature 30°C. Results: The linear range of ursolic acid by HPLC was Y=447684X-7680 (r=0.9999). The average recovery was 98.84% and RSD was 1.69% (n=6). Conclusion: The determination of ursolic acid by HPLC method is easy to operate, with high sensitivity and good reproducibility. It can provide a certain basis for the quality control of S. striate as an effective site.