论文部分内容阅读
目的 :从时间依赖性抗原呈递动力学的角度研究穿膜序列 (CPP)对 MHC- 类限制性 CTL 表位呈递的影响。 方法 :应用多肽固相合成技术 ,分别合成含 CPP与 H- 2 Kb限制性 CTL 表位 OVA2 57- 2 6 4的融合多肽 ,同时合成该表位 N、C端自然延伸的抗原肽和无关对照肽。采用流式细胞仪 (FACS)分析技术 ,检测抗原呈递细胞 (APC)在不同时相点对上述各抗原肽进行 MHC- 类呈递的情况。结果 :与不含 CPP的抗原肽相比 ,含 CPP的抗原肽中的 CTL 表位更易进入 APC内 MHC- 类呈递途径 ,表现为呈递所需时间明显缩短 (P<0 .0 5 ) ,且同一时相点的呈递强度显著增加 (P<0 .0 5 )。 结论 :在外源性抗原肽中引入穿膜序列可明显促进其 MHC- 类限制性 CTL 表位的呈递效率 ,从而有效增强外源性抗原肽的免疫原性
OBJECTIVE: To investigate the effect of transmembrane sequence (CPP) on the presentation of MHC-restricted CTL epitopes from a time-dependent antigen presentation kinetics perspective. Methods: The fusion peptide of OVA2 57-264 containing CTL epitope of CPP and H-2 Kb was synthesized by solid-phase peptide synthesis technique. At the same time, natural epitopes of N and C termini were synthesized and irrelevant Peptide. Flow cytometry (FACS) analysis was used to detect the MHC-like presentation of antigen presenting cells (APCs) at various time points. Results: CTL epitopes in CPP-containing antigenic peptides were more likely to enter the MHC-class presentation pathway in APC than did CPP-free antigenic peptides, showing a significantly shorter time to presentation (P <0.05), and The presentation intensity at the same time point increased significantly (P <0.05). CONCLUSION: The introduction of transmembrane sequence into exogenous antigen peptides can significantly improve the presentation efficiency of MHC-restricted CTL epitopes and thus effectively enhance the immunogenicity of exogenous antigen peptides