目的研究透明质酸酶(H yase)与血管内皮生长因子(VEG F)在人乳腺癌侵袭与转移中的意义。方法应用组织匀浆、ELSA及免疫组化等方法测定H yase和VEG F在人乳腺癌中的表达并对其病理特征进行比较。结果乳腺癌组织学分级Ⅰ级、Ⅱ级、Ⅲ级组H yase的均值分别为(3.99±1.91)m U/g、(8.01±2.59)m U/g和(12.00±3.96)m U/g,Ⅲ级和Ⅱ级组H yase表达明显高于Ⅰ级组,Ⅲ级组明显高于Ⅱ级组,其差异具有显著的统计学意义(P<0.05)。乳腺癌无淋巴结转移组和淋巴结转移组H yase的均值分别为(5.25±2.84)m U/g和(8.21±3.26)m U/g,乳腺癌淋巴结转移组H yase表达明显高于无淋巴结转移组。VEG F阳性率在淋巴结转移组为82.6%,明显高于无淋巴结转移组33.3%(P<0.05)。VEG F阳性组和阴性组H yase为(8.39±3.26)m U/g和(5.16±2.58)m U/g,二者间具有显著性统计学差异(P<0.05)。结论H yase和VEG F一样,与人乳腺癌侵袭与转移相关。 Objective To investigate the significance of hyaluronidase (H yase) and vascular endothelial growth factor (VEGF) in the invasion and metastasis of human breast cancer. Methods The expression of H yase and VEG F in human breast cancer were determined by histological homogenate, ELSA and immunohistochemistry, and their pathological features were compared. Results The mean H yase in grade Ⅰ, grade Ⅱ and grade Ⅲ breast cancer were (3.99 ± 1.91) m U / g, (8.01 ± 2.59) m U / g and (12.00 ± 3.96) m U / g The expression of H yase in grade Ⅲ and grade Ⅱ was significantly higher than that in grade Ⅰ, grade Ⅲ was significantly higher than that in grade Ⅱ, and the difference was statistically significant (P <0.05). The mean values ​​of H yase in breast cancer without lymph node metastasis and in lymph node metastasis were (5.25 ± 2.84) mU / g and (8.21 ± 3.26) mU / g, respectively. The expression of H yase in breast cancer with lymph node metastasis was significantly higher than that without lymph node metastasis group. The positive rate of VEG F was 82.6% in lymph node metastasis group, which was significantly higher than that in non-lymph node metastasis group (33.3%, P <0.05). The H yase of the positive and negative groups were (8.39 ± 3.26) m U / g and (5.16 ± 2.58) m U / g, respectively. There was a significant difference between the two groups (P <0.05). Conclusions H yase, like VEG F, is associated with invasion and metastasis of human breast cancer.