研究了重组ＩＬ１２、ＩＬ７、ＩＬ２协同对健康人外周血单个核细胞（ＰＢＭＣ）的体外增殖和杀瘤活性的影响。结果表明，高浓度ＩＬ２与低浓度ＩＬ２协同可引起ＰＢＭＣ明显增殖，若在该培养体系中加入低浓度的ＩＬ２，可使这种增殖效应显著增强。如果加大ＩＬ１２剂量时会产生更强的细胞毒活性，并呈现剂量依赖关系。在此培养条件下的培养上清中亦检出了较高水平的ＩＦＮγ。对培养３～１４ｄ的协同刺激细胞表型分析显示，ＣＤ５６抗原阳性细胞百分率呈递增趋势，提示ＣＤ５６＋的ＮＫ细胞构成了产生增殖和杀伤效应的主要细胞群体。 To study the recombinant IL  12, IL  7, IL  2 synergistic effect on human peripheral blood mononuclear cells (PBMC) in vitro proliferation and tumorigenicity. The results showed that high concentrations of IL 2 and low concentrations of IL 2 synergistic PBMC can cause significant proliferation, if the culture system by adding a low concentration of IL 2, can make such a proliferation effect was significantly enhanced. If you increase the IL 12 dose will produce more cytotoxic activity, and showed a dose-dependent relationship. In this culture conditions, the culture supernatant also detected a high level of IFN γ. Analysis of the co-stimulatory cell phenotypes after 3 to 14 days of culture showed that the percentage of CD56 antigen positive cells showed an increasing trend, suggesting that CD56 + NK cells constitute the major cell population that produces proliferative and cytotoxic effects.