论文部分内容阅读
作者对50例健康人骨髓进行长期培养,其中6例进行了13次培养物的定量研究。同时对贴壁细胞作了初步观察。作者将骨髓标本经850g/分离心10分钟,低渗溶解红细胞,将1500万个细胞悬浮在10～30毫升培养液中,其中含20%的马血清、10~(-4)M 2-硫甘油和青、链霉素,用 T-75光泽塑料培养瓶在37℃、5%CO_2孵箱培养,每周换液1次,换液时倒去一半培养液补以等量新鲜培养液。培养3～4周后,从同一供髓者取骨髓,用 Ficoll-Hypaque 分离液分离低密度细胞,把细胞置平皿内30分钟以除去贴壁细胞,复种在长有贴壁细胞的培养瓶中,记为“0”天。取“0”天细胞和每周1次取悬浮培养细胞,测定 CFU-GM、CFU
In the long-term culture of 50 healthy human bone marrows, 6 of them performed a quantitative study of 13 cultures. At the same time made a preliminary observation of adherent cells. Bone marrow samples were centrifuged for 10 minutes at 850 g / well, red blood cells were lysed, and 15 million cells were suspended in 10 to 30 ml of culture medium containing 20% horse serum, 10 -4 M sulfur Glycerol and cyanine, streptomycin, with T-75 glossy plastic culture flasks at 37 ℃, 5% CO 2 incubator, changing fluid once a week, half of the liquid medium replaced when replaced by the same amount of fresh broth. After 3 to 4 weeks of culture, bone marrow was collected from the same donor, low density cells were isolated using Ficoll-Hypaque separation fluid, the cells were plated in the plate for 30 minutes to remove adherent cells, and seeded in long, adherent cells in flasks , Recorded as “0” days. Take “0” days of cells and suspension cells cultured once a week to measure CFU-GM, CFU