目的:研究单用黄药子及黄药子配伍当归对大鼠肝组织grp78和bad基因表达的影响,探讨黄药子引起肝损伤以及配伍当归后其损伤减轻的机制。方法:30只清洁级SD雄性大鼠随机分为黄药子组、黄药子配伍当归组和对照组,每组10只。上述3组大鼠分别给予黄药子提取物混悬液(1mL相当于黄药子0.067g)、黄药子和当归提取物混悬液(1mL相当于黄药子0.067g,当归0.133g)及0.5%羧甲基纤维素钠(CMC)溶液各2mL,灌胃,2次/d,持续14d。末次给药24h后采用实时定量聚合酶链式反应(实时定量PCR)的方法测定各组大鼠肝脏grp78和bad基因的表达。结果:3组大鼠肝脏grp78和bad基因表达通过看家基因gapdh校正后的结果如下:黄药子组grp78和bad基因的2-△△Ct分别为339.9和256.2,黄药子配伍当归组分别为17.0和9.9,对照组均为1.0。与对照组比较,黄药子组grp78和bad基因表达明显上调,差异有统计学意义(P<0.01);而与黄药子组比较黄药子配伍当归组基因表达有显著的下调趋势,差异有统计学意义(P<0.01)。结论:黄药子可上调大鼠肝脏grp78和bad基因的表达,这可能是黄药子引起肝损伤的机制;当归对黄药子致大鼠肝脏grp78和bad基因表达上调的拮抗作用可能是其减轻黄药子所致肝损害的机制之一。 OBJECTIVE: To study the effect of xanthium and xanthium on the expression of grp78 and bad genes in rat liver tissue, and to explore the mechanism of xanthium-induced liver injury and lessened injury after compatibility with Angelica sinensis. Methods: Thirty male SD rats were randomly divided into xanthium group, xanthium group and control group, with 10 in each group. The above three groups of rats were respectively given Xanthium sibiricum extract suspension (1 mL corresponding to xanthophylls 0.067 g), xanthophylls and Angelica extract suspensions (1 mL corresponding to xanthophylls 0.067 g, Angelica sinensis 0.133 g) and 0.5% carboxymethylcellulose Sodium (CMC) solution of 2mL, gavage, 2 times / d, for 14d. After the last administration, the expression of grp78 and bad genes in liver of each group were determined by real-time quantitative polymerase chain reaction (real-time quantitative PCR). Results: The results of gapdh correction of housekeeping gene expression of grp78 and bad genes in 3 groups of rats were as follows: the 2-△△ Ct of grp78 and bad genes of xanthium group were 339.9 and 256.2, respectively, while that of xanthate group was 17.0 and 9.9 , The control group were 1.0. Compared with the control group, the expressions of grp78 and bad genes were significantly up-regulated in xanthate group (P <0.01), while the expression of grp78 and xanthomonas in xanthium was significantly down-regulated compared with xanthate group (P <0.01). CONCLUSION: Xanthium can up-regulate the expression of grp78 and bad genes in rat liver, which may be the mechanism of xanthium-induced hepatic injury. The antagonistic effect of Angelica sinensis on up-regulated expression of grp78 and bad genes in rat liver may be due to their reduced hepatic damage induced by xanthan gum One of the mechanisms.