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TGF-β信号的缺失与许多组织的恶性增生有关,Smad4是该通路的重要蛋白,其缺失或突变与多种癌症相关。本研究目的主要是探讨细胞浆信息传递介质Smad4在白血病细胞中的定位及表达。分离白血病病人骨髓单个核细胞,镜下鉴定白血病细胞数量达90%以上,应用免疫组织化学技术检测白血病细胞中Smad4蛋白的表达。结果表明Smad4蛋白主要表达于细胞核中,部分表达于胞浆中,35例白血病病人中6例急性淋巴细胞性白血病患者Smad4无表达,包括L11例,L31例,L24例;7例急性非淋巴细胞性白血病(1例M0,1例M1,2例M2a,1例M3a,1例M4b,1例M6)和1例慢性粒细胞性白血病患者白血病细胞中也无Smad4表达,其余病人白血病细胞中均表达Smad4。结论Smad4主要定位于细胞核中,在部分白血病细胞中无表达,Smad4基因或功能的改变可能与人类AML的发生有关。
Deletion of TGF-β signaling is associated with malignant hyperplasia in many tissues. Smad4 is an important protein in this pathway and its deletion or mutation is associated with a variety of cancers. The purpose of this study is to investigate the localization and expression of Smad4, a cytosolic signaling mediator, in leukemia cells. Bone marrow mononuclear cells were isolated from patients with leukemia, and the number of leukemia cells was identified more than 90% by microscopy. The expression of Smad4 protein in leukemia cells was detected by immunohistochemistry. The results showed that Smad4 protein was mainly expressed in the nucleus and partly expressed in the cytoplasm. Six of the 35 leukemia patients had no expression of Smad4, including L11, L31 and L24 cases. Seven cases of acute non-lymphocytic leukemia There was also no Smad4 expression in leukemia cells (1 M0, 1 M1, 2 M2a, 1 M3a, 1 M4b, 1 M6) and 1 chronic myelogenous leukemia Smad4 is expressed. Conclusion Smad4 mainly locates in the nucleus and is not expressed in some leukemia cells. The change of Smad4 gene or function may be related to the occurrence of human AML.