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RNA编辑是一种转录后基因加工修饰现象,广泛存在于高等植物细胞器中。已有研究表明,RNA编辑与植物发生白化或者黄化有关。通过PCR、RT-PCR及测序的方法,对具有阶段性白化特性的小麦(Triticum aestivum)返白系FA85及其野生型矮变一号(Aibian 1)的叶绿体蛋白质编码基因RNA编辑位点进行了测定,在14个基因上发现了26个编辑位点。有5个编辑位点在2个株系之间存在编辑效率的差异,且这些差异的位点均位于编码叶绿体RNA聚合酶的基因上,其中3个位点编辑前后对应的蛋白质二级结构可能有差异。对2个株系叶绿体中PEP、NEP及PEP、NEP共同依赖基因转录水平的检测显示,除psbA和clpP外,其它基因在小麦返白系中的转录水平均有不同程度的下降。这种转录水平的显著下降及叶绿体RNA聚合酶基因上RNA编辑位点编辑效率的改变,可能与小麦返白系叶片的返白有关。
RNA editing is a post-transcriptional gene processing and modification phenomenon, widely found in higher plant organelles. Studies have shown that RNA editing is associated with plant whitening or yellowing. The chloroplast protein-coding gene RNA editing sites of wheat germplasm (Triticum aestivum) with a whitened line FA85 and its wild type Aibian 1 were assayed by PCR, RT-PCR and sequencing. , Found 26 editing sites on 14 genes. There are five editing sites in the editing efficiency differences between the two strains, and these differences are located in the site coding chloroplast RNA polymerase gene, including three sites before and after editing the corresponding protein secondary structure may Differences. The transcriptional level of PEP, NEP, PEP and NEP in two chloroplast strains was significantly lower than that of psbA and clpP. The significant decrease of this transcriptional level and the editing efficiency of RNA editing sites on chloroplast RNA polymerase genes may be related to the return of wheat leaves to the white line.