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目的建立高效液相色谱-串联质谱法测定多种水果中的6种植物生长调节剂残留量的方法。方法样品经组织捣碎,采用Qu ECh ERS法预处理,采用含1%(V/V)乙酸乙腈进行提取,分散固相萃取吸附剂(无水硫酸镁和C18粉末)净化,用Agilent Extend-C18色谱柱分离,以0.01%(V/V)氨水和乙腈溶液作为流动相进行梯度洗脱,基质匹配标准溶液外标法定量。结果多效唑、氯吡脲和噻苯隆在0.2μg/L~200μg/L,赤霉素、2,4-D和4-CPA在2μg/L~500μg/L时,具有良好的线性关系,相关系数(r)均>0.995 2。6种植物生长调节剂的检出限为0.02μg/kg~1.5μg/kg,定量限为0.07μg/kg~5.0μg/kg,加标回收率为79.5%~104.9%,相对标准偏差(RSD)为4.6%~14.1%。结论该方法快速简便,定量准确,可满足多种水果中6种植物生长调节剂的残留检测要求。
OBJECTIVE To establish a method for the determination of six plant growth regulators residues in many fruits by high performance liquid chromatography-tandem mass spectrometry. Methods The samples were mash-crushed, pretreated with Qu ECh ERS method, extracted with 1% (V / V) acetic acid in acetonitrile and purified by solid-phase extraction sorbent (anhydrous magnesium sulphate and C18 powder) C18 column, gradient elution was carried out with 0.01% (V / V) aqueous ammonia and acetonitrile as the mobile phase, and the matrix was matched with standard solution by external standard method. Results There was a good linear relationship between paclobutrazol, clopidogrel and thidiazuron at 0.2μg / L ~ 200μg / L, gibberellin, 2,4-D and 4-CPA at 2μg / L ~ 500μg / L (R)> 0.995. The detection limit of 2.6 plant growth regulators was 0.02μg / kg ~ 1.5μg / kg, the limit of quantification was 0.07μg / kg ~ 5.0μg / kg, the recovery was 79.5% ~ 104.9%, and the relative standard deviation (RSD) was 4.6% ~ 14.1%. Conclusion The method is rapid, simple and accurate. It can meet the requirements of 6 kinds of plant growth regulators in many fruits.