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目的探讨微小核糖核酸-21(mi R-2)在乳腺癌中的表达及其与乳腺癌侵袭转移的关系。方法选取2014年2月至2016年2月湖南中医药高等专科学校附属第一医院收治的65例乳腺癌患者为观察组,其中存在淋巴结转移39例,未发生淋巴结转移26例;并以同期行健康体检的65例健康成人及收治65例乳腺良性病变患者作为对照A组与对照B组。分别对采集的血清标本与乳腺细胞依次开展mi R-21临床检验,评估其在乳腺癌中的表达并分析与乳腺癌侵袭转移的相关性。结果观察组患者的mi R-21相对表达量明显高于A组与B组,差异有统计学意义(P<0.05);观察组中发生淋巴结转移的患者mi R-21相对表达量明显高于无淋巴结转移患者,差异有统计学意义(P<0.05);MCF-7、MDA-MB-231与MDA-MB-468乳腺癌细胞的mi R-21相对表达量均明显高于HBL-100组,且MDA-MB-231与MDA-MB-468侵袭性乳腺癌细胞的mi R-21相对表达量明显高于MCF-7非侵袭性乳腺癌细胞,差异均有统计学意义(均P<0.05);转染mi R-21抑制剂的MDA-MB-231侵袭性人乳腺癌细胞迁移至小室细胞数量与穿过基质胶细胞数量均明显低于A组乳腺细胞,组间比较差异均有统计学意义(均P<0.05)。结论乳腺癌患者的mi R-21呈高表达状态,其表达水平同癌细胞侵袭转移具有相关性,抑制mi R-21水平有利于控制肿瘤细胞转移。
Objective To investigate the expression of mi R-2 in breast cancer and its relationship with invasion and metastasis of breast cancer. Methods Sixty-five breast cancer patients admitted to the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine between February 2014 and February 2016 were selected as the observation group. There were 39 cases with lymph node metastasis and 26 cases without lymph node metastasis. A total of 65 healthy adults undergoing physical examination and 65 patients with benign breast lesions were selected as control group A and control group B. The mi R-21 clinical tests were performed on the collected serum samples and breast cells in order to evaluate their correlation with breast cancer invasion and metastasis. Results The relative expression of mi R-21 in observation group was significantly higher than that in group A and B (P <0.05). The relative expression of mi R-21 in observation group was significantly higher than that in group with lymph node metastasis The expression of mi R-21 in MCF-7, MDA-MB-231 and MDA-MB-468 breast cancer cells were significantly higher than those in HBL-100 group (P <0.05) , And the relative expression of mi R-21 in MDA-MB-231 and MDA-MB-468 invasive breast cancer cells was significantly higher than that in MCF-7 non-invasive breast cancer cells (all P <0.05 ). The number of migrating MDA-MB-231 invasive breast cancer cells transfected with mi R-21 inhibitor and the number of cells passing through the stromal cells were significantly lower than those in group A, with statistically significant differences between the two groups Significance (both P <0.05). Conclusion The expression of mi R-21 in breast cancer patients is highly expressed. The expression of mi R-21 is correlated with the invasion and metastasis of cancer cells. Inhibition of mi R-21 may be helpful to control tumor metastasis.