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目的 :观察甘草对P-糖蛋白(P-gp)底物经大鼠不同区段肠黏膜透过性的差异,以评价其对肠黏膜P-gp功能的影响。方法:通过对大鼠口服甘草煎液、生理盐水一周后,使用体外扩散池(Ussing Chamber)技术评价罗丹明123(R123)和荧光素钠(CF)经大鼠空肠回肠结肠黏膜的经时吸收方向和分泌方向的透过量和表观渗透系数。R123和CF在接受室的浓度用荧光分光光度计检测。结果:R123的透过性比较中,甘草组与生理盐水组组内比较,差异有统计学意义(F=5.602,P=0.000)。CF的透过性比较中,甘草组组内比较,差异有统计学意义(F=20.140,P=0.000)。结论:甘草对底物药物R123的影响可能与存在于肠黏膜的P-gp有关,这可能是甘草与药物产生协同作用的机制之一。
OBJECTIVE: To observe the difference of intestinal mucosal permeability of P-glycoprotein (P-gp) substrate in different sections of rat to evaluate its effect on the function of P-gp in intestinal mucosa. Methods: Ussing Chamber was used to assess the time-dependent absorption of rhodamine 123 (R123) and sodium fluorescein (CF) through the jejunal mucosa of rats’ jejunum after rats were orally administered licorice root decoction and saline for one week. Direction and secretion direction of the permeability and apparent permeability coefficient. The concentrations of R123 and CF in the receiving compartment were measured with a fluorescence spectrophotometer. Results: In the comparison of permeability of R123, the difference was statistically significant (F = 5.602, P = 0.000) in licorice group and saline group. Comparing the permeability of CF, licorice group within the group, the difference was statistically significant (F = 20.140, P = 0.000). Conclusion: The effect of licorice on R123 may be related to the presence of P-gp in intestinal mucosa, which may be one of synergistic mechanism of licorice and drug.